Cell death assaysDetection of DNA fragmentation (SubG1 cells) was performed in propidium iodide stained cells as previously described . In vitro determination of cytoplasmic histone-associated-DNAfragments (mono- and oligonucleosomes) was performed…
3. Molecular Docking Studies3.1. Preparation of Ligands and Receptor. The NCI and ChemBridge database libraries were chosen for virtual screening of small-molecule inhibitors on the basis of structure similarity searches.…
These ligands were docked onto the binding pocket of the PDB file 3C45 with eHiTS v2009 (SimBioSys Inc., Toronto, Canada; http://www.simbiosys.ca/ehits) using default docking conditions, with the exception of the…
Assessment of Potential Off-target Activity of 4bIn addition to the HDAC profiling performed, we also queried 4b for any apparent potential off-target effects, which could complicate any finding of efficacy…