The pyrosequencing primer contained in the assay, complementary to the single-stranded template, was then hybridized to the template, and the pyrosequencing reaction was executed by the sequential addition of one nucleotides in a predefined get
he Bacterial Artificial Chromosome (BAC) clones, RP11367F29, and RP1183L6 which span the SYK gene area at 9q22.2, were obtained from the BAC/PAC Useful resource at Children's Healthcare facility Oakland Research…