For every single band, the OD of expression degrees of StAR protein have been quantified (arbitrary models) and normalized to the corresponding III Complex protein
Cells had been transiently transfected using Lipofectamine 2000 reagent and transfection was done with pRc/CMVi plasmid both that contains StAR wild-sort cDNA (StAR wt) or StAR mutated cDNA (StAR S232A),…