NCP and PT-DP, diluted one/twenty five, have been incubated for twenty min with an activation mix containing possibly thrombin-thrombomodulin intricate (last thrombin focus 8000 nM) or MASP-one at final concentrations of fifty mg/ml (1100 nM)

Direct activation of C3 by MASP-one takes place with really minimal catalytic efficiency and is not of physiological relevance . However, MASP-1 has been recommended to act synergistically with MASP-two…