To stain cells positive for alkaline phosphatase, cells were first fixed by incubating them for 20 minutes at room temperature
Up coming, cells ended up blocked and permeabilized by a 20-minute incubation with PBS-BS (PBS+.five% BSA+.05% saponin) and then incubated with mouse anti-TRA-one-sixty antibody (Chemicon, diluted 1/100 in PBS-B (PBS+.five%…