Product Name :
Proteasome 20S assay kit
Sequence:
Purity:
Molecular Weight:
Solubility :
Appearance:
Use/Stability :
Description:
A QUANTIZYME® Assay System. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome. The kit includes purified, human erythrocyte 20S proteasome (20µg). The Suc-LLVY-AMC fluorogenic peptide substrate supplied with this kit is a proteasome substrate commonly used for measuring chymotrypsin-like peptidase activity. The detection of proteolytic activity is based on the release of free AMC fluorophore and offers the following advantages: non-radioactive, convenient real-time kinetics of substrate cleavage, and excellent sensitivity. A proteasome inhibitor, epoxomicin, is included as a test inhibitor for screening purposes. Proteasome 20S assay kit Fig4A Proteasome 20S assay kit Standard curve Proteasome 20S assay kit Fig3A Proteasome 20S assay kit Fig3B Proteasome 20S assay kit Fig4B Proteasome 20S assay kit time_courses Proteasome 20S assay kit Fig4A Proteasome 20S assay kit Standard curve Proteasome 20S assay kit Fig3A Proteasome 20S assay kit Fig3B Proteasome 20S assay kit Fig4B Proteasome 20S assay kit time_courses
CAS :
Solubility:
Formula:
Additional Information :
| Application Fluorescent detection, HTS | Contents Proteasome 20S (human) (purified) Suc-Leu-Leu-Val-Tyr-AMC Proteasome Assay Buffer Sodium Dodecyl Sulfate Epoxomicin AMC (7-Amino-4-methylcoumarin) Calibration Standard 1/2 Volume White Microplates