Product Name :
Nerve growth factor 7S (mouse)
Sequence:
Purity:
≥98% (HPLC)
Molecular Weight:
~130kDa.
Solubility :
Appearance:
Use/Stability :
As indicated on product label or CoA when stored as recommended. Stable for 2-3 weeks when stored at room temperature. After reconstitution, store for up to one week at +4°C and for 4-6 weeks at -80°C.
Description:
CAS :
Solubility:
Formula:
Additional Information :
| Alternative Name NGF 7S | Biological Activity Bioassayed for neurotrophic activity using the rat pheochromocytoma cell PC12 cultures over a period of 7-14 days. Neurotrophic activity is in the range of 0.5-50ng/ml. Recommended concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100ng/ml medium. | Formulation Lyophilized.{{2762552-74-7} web|{2762552-74-7} Protocol|{2762552-74-7} Formula|{2762552-74-7} custom synthesis} | MW ~130kDa.{{1614-12-6} web|{1614-12-6} Purity & Documentation|{1614-12-6} References|{1614-12-6} manufacturer} | Purity ≥98% (HPLC) | Quantity Determined by Lowry and Pierce method.PMID:30521186 | Reconstitution Dilute in sterile water or DMEM to a concentration of at least 10µg/ml and store at -80°C before use. Medium containing NGF cannot be reused due to NGF degradation. | Source Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4, 79 (1984). | UniProt ID P01139