Ntal biomarkers into the atmosphere. Here, we characterize the neural cells derived-EV and hypothesize that impaired EV expression can disrupt cell survival communications in the course of neuroinflammation. Approaches: The part of HSPB-EV in inflammation is investigated in two actions. (1) Establish HSPBs expressing neural cell lines for the production of HSPB-EV. (2) Isolation and characterization of EV working with distinct tactics. Outcomes: NTA measurement of microglial derived-EV showed an enhanced EV secretion upon inflammation. Stable HSPBs expressing cell derived-EV revealed a decrease in EV release through inflammation. SPR evaluation of oligodendrocyte derived-EV showed interactions with ICAM1 and HSP70. Furthermore, immunoblot evaluation of oligodendrocyte derived-EV showed a downregulation of monomeric HSPB8 and phosphorylated HSPB1 for the duration of inflammation. Summary/Conclusion: Neural cells derived-EVs constitutively express HSPB1 and HSPB8. Nonetheless upon inflammation, there’s a downregulation of each the monomeric types too as the phosphorylated HSPB1. This study shows that reduced expression inside the extracellular HSPB1/B8-EV upon neuroinflammation can impair neural cell survival signalling. Funding: This work was financed by Hasselt University and by EFRO via the Interreg V Grensregio Vlaanderen Nederland project Trans Tech Diagnostics.ISEV 2018 abstract bookSymposium Session 29 Late Breaking Abstracts Chair: Tang Extended Shen Location: Area 5 09:000:LB02.On-chip liquid biopsy: progress in isolation of exosomes and their RNA sequencing for prognosis of prostate Delta-like 4 (DLL4) Proteins Recombinant Proteins cancer Navneet Dogra1; Gustavo Stolovitzky2; Carlos Cordon3; Ashutosh Tewari3; Kamlesh Yadav1; Russell McBride4; Eren Ahsen Mehmet5; Stacey Gifford6; Benjamin Wunsch7; Joshua Smith7; Sungcheol Kim7 Icahn College of Medicine at Mount Sinai, New York City, USA; 2IBM/Icahn College of Medicine at Mt. Sinai, New York, USA; 3Icahn College of Medicine at Mt. Sinai, New York, USA; 4Icahn College of Medicine at Mount Sinai IBM, NY, USA; 5Icahn School of Medicine at Mount Sinai, NY, USA; 6IBM, New York, USA; 7IBM, NY, USABackground: Exosomes are an fascinating target for “liquid biopsies”. On the other hand, isolation of exosomes and reproducible detection of their biomarkers remains an ongoing challenge. We have created a microfluidic Cathepsin D Proteins Synonyms nanoscale DLD (deterministic lateral displacement) device that brings capabilities with size based sorting of colloidal particles at the tens of nanometres scale (Wunsch et al., Nat Nanotechnol. 2016). Methods: Utilizing our chip technologies, we’ve isolated exosomes from prostate cancer cell lines and patient tissue, blood and urine samples. After exosome isolation, small RNA libraries were ready and sequencing is carried out at New York Genome Center making use of illumine sequencer HISeq2500. Our nanofluidic pillar array is manufactured in SiO2 mask applying optical contact lithography and deep ultra violet lithography. Benefits: We demonstrate microfluidic on-chip size-based separation of exosomes. We showed that our microfluidic device is capable of sorting exosomes population from distinctive bodily fluids and cell culture medium. As soon as exosomes are isolated through the chip, we performed RNA sequencing study for biomarker discovery. These outcomes are vital and fascinating for the following motives: (1) we have been capable to separate smaller sized particles (beneath 30 nm) from bigger (3000 nm) vesicle population. (2) Exosomal RNA content material from the prostate cancer patient urine and blood samples have been compa.