Rabbit anti-IGFBP1 Polyclonal Antibody

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Product Details FAQ Citations(1) Video Pictures Documents |Overview |Description This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. |Synonym Insulin-like growth factor-binding protein 1, IBP-1; IGF-binding protein 1, Placental protein 12, PP12 |Host Rabbit |Specificity IGFBP1 Antibody detects endogenous levels of total IGFBP1. |Species Reactivity Human |Applications WB 1:500-1:2000, IHC 1:50-1:100, ELISA(peptide) 1:20000-1:40000 |Immunogen A synthesized peptide derived from Human IGFBP1(Accession P08833), corresponding to amino acid residues W229-V257. |Properties |Form Liquid |Concentration 1mg/ml |Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |Clonality Monoclonal Antibody |Clone 196-47 |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |Target |Background Insulin-like growth factor binding proteins (IGFBPs) are a family of proteins binding to Insulin-like growth factors (IGFs), generally including IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, and IGFBP6 [PMID: 30214426]. IGFBP1, a 40 to 50 kDa protein, which plays an indispensable role in normal growth and development, and in the pathophysiology of various tumors. IGFBP-1 has been shown to be associated with the risk of various tumors, and has a vital function in regulating tumor behaviors such as proliferation, migration, invasion and adhesion through different molecular mechanisms. The biological actions of IGFBP-1 in cancer are found to be related to its phosphorylation state, and the IGF-dependent and -independent mechanisms. [PMID: 1648311, PMID: 33841624]. |Post-translational modifications Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis. |Cellular localization Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus; |UniProt P08833 | Western blot analysis of extracts of Mouse livertissue lysate, using IGFBP1antibody|Immunohistochemistryof paraffin-embeddedHuman liver cancer tissue tissue usingIGFBP1antibody. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.