Product Name :
Anti-GFP, AlpSdAbs® VHH(iFluor488)
Applications:
ELISA,ICC/IF,Flow Cyt
Reactivity :
GFP
Conjugate:
iFluor488
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-GFP, AlpSdAbs® VHH(iFluor488) is designed for detecting GFP fusion proteins. Anti-GFP, AlpSdAbs® VHH(iFluor488) is based on monoclonal, recombinant, single domain antibody to GFP coupled to iFluor488 . Based on immunoelectrophoresis and/or ELISA, Anti-GFP, AlpSdAbs® VHH(iFluor488) detects the GFP selectively, no reactivity with other proteins. | Immunogen: GFP | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c | Conjugate: iFluor488(Ex: 495nm, Em: 519nm), 1 moles iFluor488 per mole VHH | Specificity: GFP | Cross-Reactivity: Recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants. Does not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP or their most common derivatives | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.{{142234-85-3} site|{142234-85-3} Protocol|{142234-85-3} Purity|{142234-85-3} supplier} 5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light | Background:Green fluorescent proteins (GFPs) and the variants thereof are widely used to study protein function and location.{{2687970-96-1} web|{2687970-96-1} Technical Information|{2687970-96-1} Data Sheet|{2687970-96-1} manufacturer} GFP was originally derived from the jellyfish Aequorea victoria. It has 238 amino acid residues and a green fluorophore, which is comprised of only three amino acids: Ser65-Tyr66-Gly67. The stable protein structure is formed by beta sheets, which have a conformation that makes up an 11-stranded drum-like structure. The stability of GFP allows it to withstand pH levels ranging from mildly acidic (pH=5.5) to extremely basic (pH=12), and can also resist temperatures of up to 65°C. GFP has major and minor excitation peaks at wavelengths of 395 nm and 475 nm, respectively.VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(
Description2 :
Anti-GFP, AlpSdAbs® VHH(iFluor488) is designed for detecting GFP fusion proteins. Anti-GFP, AlpSdAbs® VHH(iFluor488) is based on monoclonal, recombinant, single domain antibody to GFP coupled to iFluor488 .PMID:20301551 Based on immunoelectrophoresis and/or ELISA, Anti-GFP, AlpSdAbs® VHH(iFluor488) detects the GFP selectively, no reactivity with other proteins.
Immunogen:
GFP
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Function:
ELISA: 1:5000 -1:20000Flow Cyt: 1:200-1:2000ICC/IF: 1:200-1:2000Super-resolution microscopyDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.