Ned from the bands of the marker (72200 kDa). The estimated sizes with the bands had been as follows: 1, 2 (4.0 kDa), 3 (38.0 kDa), 4 (14.0 kDa), 5 (9.five kDa), 6 (8.0 kDa), and 7 (four.7 kDa). (B) Representative SELDI-TOF-MS spectra from the low-molecular-weight proteins (52 20 kDa) in the extracts. The x-axis represents the m/z values, plus the y-axis represents the intensity on the signals (mA). Peaks with signal/noise ratios (S/N) .5 were automatically detected. doi:ten.1371/journal.pone.0102509.gPLOS One | www.plosone.orgBioactivity Evaluation and Chemical Profiling of Lignosus rhinocerotisTable four. Chemical constituents in LR-MH and LR-MT based on GC-MS analysis.RT (min) CompoundsMolecular formula Molecular weight Location ( ) LR-MH LR-MT ND 4.46 10.78 six.50 7.ten 11.23 14.52 1.25 1.40 0.53 0.23 3.Atazanavir 54 16.98 0.27 1.12.12 12.97 13.51 14.91 15.30 16.56 16.97 17.19 17.39 18.35 18.70 18.93 21.51 22.60 25.Methyl b-D-galactopyranoside Arabinitol Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydroHexadecanoic acid, methyl ester n-Hexadecanoic acid 9,12-Octadecadienoic acid (Z,Z)-, methyl ester 9,12-Octadecadienoic acid (Z,Z)Octadecanoic acid Hexadecanamide 11,13-Eicosadienoic acid, methyl ester Cyclopentadecanone, 2-hydroxy9-Octadecenamide, (Z)9,12-Octadecadienoic acid (Z, Z)-, 2-hydroxy-1-(hydroxymethyl)ethyl ester two,3-Dihydroxypropyl elaidate ErgosterolC7H14O6 C5H12O5 C7H10N2O2 C17H34O2 C16H32O2 C19H34O2 C18H32O2 C18H36O2 C16H33NO C21H38O2 C15H28O2 C18H35NO C21H38O4 C21H40O4 C28H44O194.18 152.15 154.17 270.45 256.42 294.47 280.45 284.48 255.44 322.53 240.38 281.48 354.52 356.54 396.eight.16 ND eight.36 1.23 6.14 two.63 11.73 ND ND ND ND four.76 13.26 0.28 NDArea ( ) was determined based on the TIC of LR-MH (Figure S1) and LR-MT (Figure S2). Identification of the compounds was based on mass spectral evaluation. RT, retention time; ND, not detected. doi:ten.1371/journal.pone.0102509.t20 and 200 mg/ml. The percentage of cell viability immediately after 72 h of incubation was determined by the following equation: Cell viability bsorbance of treated cells=absorbance of untreated cells|Determination of chemical composition from the extractsTotal sugars have been determined utilizing the phenol-sulphuric assay [22].Bromothymol Blue D-glucose (Merck) was utilized as the normal.PMID:24324376 Protein content was analysed applying the Pierce Coomassie Plus (Bradford) Protein Assay (ThermoScientific, Massachusetts, USA) according to the manufacturer’s protocol, with bovine serum albumin because the typical. The level of phenolics was estimated using the FolinCiocalteu reagent [23] with gallic acid (Sigma-Aldrich, USA) because the standard.Table five. Chemical constituents in LR-BH and LR-BT primarily based on GC-MS analysis.RT (min)CompoundsMolecular formulaMolecular weightArea ( ) LR-BH LR-BT 0.65 0.51 ND ND 20.12 four.18 ND ND ND 3.19 1.3.61 four.46 six.70 11.16 13.68 15.19 15.23 16.95 17.36 18.93 19.2-Furancarboxaldehyde, 5-methylBenzeneacetaldehyde 1,four:three,6-Dianhydro-a-D-glucopyranose b-D-glucopyranose, 1,6-anhydro Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydroPyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)n-Hexadecanoic acid 9,12-Octadecadienoic acid (Z,Z)Hexadecanamide 9-Octadecenamide, (Z)Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)-C6H6O2 C8H8O C6H8O4 C6H10O5 C7H10N2O2 C11H18N2O2 C16H32O2 C18H32O2 C16H33NO C18H35NO C14H16N2O110.11 120.15 144.13 162.14 154.17 210.27 256.42 280.45 255.44 281.48 244.0.89 1.24 2.08 0.80 15.60 ND 2.86 2.63 2.50 7.43 NDArea ( ) was determined based on the TIC of LR-BH (Figure S3) and LR-BT (Figure S4). Identificati.