es. Apoptosis mediated by mitochondrial harm is suggested to become associated with AFB1-induced oxidative anxiety induced by AFB1. In our study, Res supplementation inhibited the expression levels of pro-apoptotic genes (e.g., Bax, P53, Caspase3, Caspase9, Cyt-C) and improved the expression levels of the anti-apoptotic gene Bcl-2 in hepatocytes, which demonstrated that dietary Res implemented the efficient inhibition of hepatocyte apoptosis brought on by AFB1. five. Conclusions This study showed that the supplementation of 500 mg/kg of Res in ducks’ diets inhibited the degree of acute liver injury of ducks exposed to AFB1, and dietary Res correctly alleviated the apoptotic pathway by inhibiting the metabolism of AFB1, up-regulating the activity of phase-II metabolism enzymes, activating the expression of Nrf2 and Sirt1, down-Animals 2021, 11,15 ofregulating the activities of NF-B, and suppressing the cell apoptosis pathway (Figure 10). Thus, Res may perhaps serve as a prospective phytochemical for use inside the therapy of acute Animals 2021, 11, x FOR PEER Critique AFB1 toxicity in ducks and also other animals.17 ofFigure 10. Res activated the Nrf2 pathway and inhibited mitochondrial apoptosis pathway to alleviate liver damage inFigure duced by AFB1. 10. Res activated the Nrf2 pathway and inhibited mitochondrial apoptosis pathway toalleviate liver damage induced by AFB1.Supplementary Materials: The following are obtainable on the net at mdpi/article/10 F.L.; w mal evaluation, S.J.; resources, X.F.; data curation, Y.W.; writing–original draft preparation, .3390/ani11123516/s1, Figures S1: editing, X.F.; supervision, X.F.; project administration, M.L. as well as a.S.; funding ing–review and Western Blot Figures.quisition, X.F. All authors have study and agreed towards the published version of the manuscript.Author Contributions: Conceptualization, F.L. and X.F.; methodology, Y.W.; Nav1.2 Compound validation, X.Z.; fAuthor Contributions: Conceptualization, F.L. and X.F.; methodology, Y.W.; validation, X.Z.; formal Funding: This function was supported by the National draft preparation, F.L.; writing– analysis, S.J.; resources, X.F.; information curation, Y.W.; writing–original Organic Science Foundation of China (317726 32072768). assessment and editing, X.F.; supervision, X.F.; project administration, M.L. and a.S.; funding acquisition, X.F. All authors have study and Review for the published version of was manuscript. Institutional agreed Board PPARβ/δ Synonyms Statement: The study the conducted in line with the recommendations ofpractices outlined by the National the Care and Use of Agricultural Animals in Agriculture R Funding: This operate was supported within the Guide for Organic Science Foundation of China (31772638, search and Teaching of Northeast Agricultural University (Protocol quantity: NEAU-(2011)-9). 32072768). Informed Consent Statement: This investigation will not be related to people today. Not applicable.Institutional Evaluation Board Statement: The study was carried out in accordance with the guidelines of the practices outlined Information Availability Statement: The rawAgricultural Animals in Agriculture Researchrequest fro in the Guide for the Care and Use of data presented within this study are accessible on the corresponding author. and Teaching of Northeast Agricultural University (Protocol number: NEAU-(2011)-9).Conflicts of Interest: The authors related to persons. Not applicable. Informed Consent Statement: This analysis is notdeclare no conflict of interest.References Data Availability Statement: The raw information presented within this study are available on requ