S had a correlation with these peaks in a comparative study on Raman spectrum. Interestingly, this concordance was constant with immunoblotting benefits. The protein markers which had uniquely overlapping peaks showed larger expression on cancerous exosomes. This outcome indicates that these proteins could contribute to the Raman spectrum of cancerous exosomes. Summary/conclusion: In conclusion, we compared exceptional Raman spectrum of lung cancer cell-derived exosomes and their protein markers. We estimated exclusive Raman spectral peaks and in comparison with Raman spectra of five protein markers. Lastly, we could determine the protein markers probably contributing for the Raman spectrum of your cancerous exosomes. Funding: This investigation was supported by a grant from the Korea Wellness Technology R D Project through the Korea Overall health Sector Development Institute, funded by the Ministry of Overall health Welfare, Republic of Korea (Grant Nos. HR14C0007).OWP2.Improvement of high CCR2 Antagonist supplier sensitivity flow cytometry for sizing and molecular profiling of individual extracellular vesicles down to 40 nm Ye Tian1; Manfei Gong1; Haisheng Liu1; Wenqiang Zhang1; Ling Ma2; Shaobin Zhu2; Xiaomei Yan1Department of Chemical Biology, Xiamen University, Xiamen, China; NanoFCM Inc., Xiamen, ChinaOWP2.05 = PF01.Comparative evaluation of Raman signals involving non-small cell lung cancer (NSCLC) cell derived exosomes and their prospective protein markers Hyunku Shin1; Hyesun Jung2; Jaena Park1; Sunghoi Hong3; Yeonho ChoiDepartment of Bio-convergence Engineering, Korea University, Seoul, Republic of Korea, Seoul, Republic of Korea; 2School of Biosystem and CaMK III Inhibitor manufacturer Biomedical Science, Department of Public Health Sciences, Korea University, Seoul, Republic of Korea;3School of Biosystem and Biomedical Science, College of Well being Science, Korea University, Seoul, Republic of Korea; 4School of Biomedical Engineering, Korea University, Seoul, Republic of KoreaBackground: Surface proteins of exosomes are of excellent interest for cancer diagnosis. Surface-enhanced Raman spectroscopy (SERS) is amongst the beneficial solutions for investigating the surface proteins. Right here, weBackground: Even though of fantastic value, sizing and molecular profiling of person extracellular vesicles (EVs) are technically difficult because of their nanoscale particle size, minute quantity of analytes, and overall heterogeneity. Our laboratory has created higher sensitivity flow cytometry (HSFCM) that enables light scattering detection of single silica nanoparticles (SiNPs) and viruses as little as 24 and 27 nm in diameter, respectively. Here we report a HSFCM-based method for quantitative multiparameter evaluation of single EVs down to 40 nm. Methods: EVs had been extracted from cell cultured medium and human blood samples by ultracentrifugation. Employing SiNPs as the size reference standards and upon refractive index mismatch correction determined by the Mie theory, precise sizing of EVs could be obtained by direct measurement in the scattered light from individual EVs. The subpopulation of EVs expressing certain surface proteins had been analyzed upon immunofluorescent staining and single particle enumeration by the HSFCM. Lipid dyes for instance PKH 26 and Dil, and nucleic acid dyes including SYTO 9 and RNA Select were also utilized to stain the EVs. The glycoproteins on the surface of single EVs were quantified by means of metabolic incorporation of azide-modified monosaccharides which have been then chemoselectively coupled to complementary alkyne-functionalized fluorophores. R.