Ed in oMSCs treated with yEVs. With respect to pluripotency markers at RNA level, a statistically significant reduce of pluripotency markers (Nanog and Oct4) in yMSCs treated with oEVs plus a statistically substantial improve in oMSCs treated with yEVs were obtained. Around the contrary, it was obtained with respect Vinculin expression. At proteomic level, a statistically substantial enhance of three isoforms of Lamin A/C in yMSCs treated with oEVs and statistically substantial lower in oMSCs treated with yEVs were obtained. Summary/conclusion: MSC-derived EVs and their self-renewal capacity have been influenced by age, as reveled by changes within the expression of self-renewal markers including Nanog, Oct4, Vinculin and Lamin A/C. These findings are essential to the recognize the influence in the ageing on MSCs and to advance inside the development EV-based therapies.PT06.Ex vivo expansion of umbilical cord blood-derived haematopoietic stem cells working with extracellular vesicles of bone marrow stromal origin Corina Ghebes1; Jess Morhayim2; Marion Kleijer1; Bram van der Eerden3; Jeroen van de Peppel3; Eric Braakman2; Carlijn Voermans1 Division of Hematopoiesis, Sanquin Investigation, Amsterdam, The Netherlands; 2Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands; 3Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, The NetherlandsPT06.Functionality of bone marrow mesenchymal stem cell-derived extracellular vesicles on self-renewal Juan Antonio Fafian Labora1; Miriam Morente-L ez2; Patricia D zBarreiro2; Pablo Fernandez-Pernas2; Mar Arufe Gonda1 Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina y ADAMTS16 Proteins Biological Activity Fisioterapia, Facultade Complement Receptor 1 Proteins custom synthesis 1Grupo de Terapia Celular y Medicina Regenerativa (TCMRCHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina y Fisioterapia, Facultade de Oza, Universidade da Coru (UDC), As Xubias, A Coru , Spain. Oza, Universidade da Coru (UDC), As Xubias, A Coru , Spain., A Coru , Spain; 2Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina, A Coru , SpainBackground: Mesenchymal stem cells (MSCs) are extremely relevant for regeneration of mesoderm tissues. The promising part of MSCs in cellbased therapies and tissue engineering is restricted due to a decline pluripotency, proliferation and immunogenic potential with increasing donor age. Extracellular vesicles (EVs) have already been recognizing as potent cars of intercellular communication on distinct biological systems. MSC-derived EVs present positive aspects over cell-based therapy since it eliminates the security concerns connected with injection of MSCs in individuals and especially beneficial for enhancing recovery from different diseases. Here, the influence of ageing on self-renewal functionality MSC-derived EVs applying an in vitro model was studied. Techniques: Two age groups from bone marrow MSCs of male Wistar rats were employed, old (three months old) and young (14 days old). EVs wereBackground: Ex vivo expansion of umbilical cord blood (UCB)-deri.