. Hence, this study aimed (i) to investigate the postharvest fungal ailments
. As a result, this study aimed (i) to investigate the postharvest fungal diseases of sweet PF-06454589 Technical Information potato in Korea and characterize the causal fungal isolates by molecular phylogenetic and morphological analyses and pathogenicity assay, and (ii) to test in vitro biocontrol activity against the fungal pathogens by two T. harzianum strains. 2. Supplies and Methods 2.1. Fungal Isolation Sweet potato storage root samples had been collected from the market of three unique areas in Korea in 2021, and also the places have been Buan-gun, Cheonan-si, and Haenamgun. Samples were kept in polyethylene bags, brought towards the laboratory, and stored in a refrigerator before isolation of pathogens. For the isolation, diseased storage roots were surface sterilized with 1 NaOCl remedy for 5 min, washed three times with sterilized distilled water, and after that air-dried on filter paper in a laminar airflow chamber. The storage roots had been placed onto potato dextrose agar (PDA) supplemented with 50 mL-1 of streptomycin, rifampicin, and kanamycin (MB cell, Seoul, Korea) to stop bacterial growth. Soon after incubation at 25 C for 30 days, person hyphal recommendations of the creating fungal colonies had been placed onto PDA and additional incubated for 50 days for culture purity. Ultimately, representative isolates were selected, assigned an identification quantity (CMML21 to CMML217), and preserved inside the Molecular Microbiology Lab, Dept. of Integrative Food, Bioscience and Biotechnology, Chonnam National University, 2-Bromo-6-nitrophenol Data Sheet Gwangju, Republic of Korea (Table S1). The fungal isolates had been preserved in 20 glycerol stock resolution at -80 C. The photographs of symptomatic sweet potato postharvest ailments and fungal colonies grown in the surface sterilized storage roots are shown in Figure 1.J. Fungi 2021, 7, 927 J. Fungi 2021, 7,3 of 18 3 ofFigure 1. Symptoms of sweet potato postharvest diseases collected from local markets in Korea: (A) Figure 1. Symptoms of sweet potato postharvest illnesses collected from local markets in Korea: Fusarium surface rot, (B) charcoal rot, (C) Aspergillus mold, (D) surface rot, (E) end rot, and (F) Pen (A) Fusarium surface rot, (B) charcoal rot, (C) Aspergillus mold, (D) surface rot, (E) end rot, and icillium mold. The surface sterilized storage root tissues of Fusarium rot, charcoal rot, along with other (F) Penicillium mold. The surface sterilized storage root tissues of Fusarium rot, charcoal rot, and diseases were placed on potato dextrose agar media containing antibiotics. Examples of fungal col other illnesses were placed on potato dextrose agar media containing antibiotics. Examples of fungal onies grown from the tissues of Fusarium rot (G,H) and charcoal rot (I) are shown. colonies grown from the tissues of Fusarium rot (G,H) and charcoal rot (I) are shown.two.2. DNA Extraction, PCR Amplification, and Sequencing two.two. DNA Extraction, PCR Amplification, and Sequencing To confirm the identity in the fungi, total genomic DNA was extracted directly from To confirm the identity on the fungi, total genomic DNA was extracted directly from the mycelia grown on PDA making use of the CTAB DNAextraction strategy [24]. Distinct gene the mycelia grown on PDA utilizing the CTAB DNA-extraction method [24]. Different gene regions, the internal transcribed spacer (ITS), elongation element 1alpha (EF1, calmod regions, the internal transcribed spacer (ITS), elongation issue 1-alpha (EF1, calmodulin ulin (CAL), RNA polymerase II second largest subunit (RPB2), modest subunit (SSU, significant (CAL), RNA polymerase I.