In injury. (C) ARKO mice show afterafter TBI.The quantitative information of GFAP level at 4 hat 4 h following brain injury. (C) ARKO mice show TBI-induced GFAP expression enhancement compared 24 h after TBI. (D) TBI. (D) QuantiTBI-induced GFAP expression enhancement compared with WTwith WT 24 h soon after Quantitative data tative information of Aztreonam Autophagy GFAPhlevel at 24 hTBI. All data are Sutezolid Formula presented because the imply normal common erof GFAP level at 24 following following TBI. All data are presented as the imply error. NS, no ror. NS, no considerable distinction; p 0.01, and p 0.001; n = 3 in each group. considerable distinction; p 0.01, and p 0.001; n = 3 in each and every group.Molecules 2021, 26, 6250 Molecules 2021, 26, x FOR PEER REVIEW5 of 16 five ofFigure three. Androgen receptor knockout increases the TBI-induced GFAP expression about thethe Figure 3. Androgen receptor knockout increases the TBI-induced GFAP expression about cortical injury internet site. (A) Illustrations on the regions of interest (white places) the mice brain after TBI cortical injury website. (A) Illustrations in the regions of interest (white regions) ofof the mice brain following TBI are shown in left panel. WT ARKO mice were performed with TBI or sham, and after that stained are shown in left panel. WT and and ARKO mice had been performed with TBI or sham, after which stained with immunofluorescence of GFAP. The GFAP cells had been indicated by white white arwith immunofluorescence of GFAP. The GFAP constructive positive cells were indicated byarrowhead. rowhead. ARKO mice showed the cells of GFAP of GFAP expression. Blue colour, DAPI (4,6ARKO mice showed the increasingincreasing cellsexpression. Blue color, DAPI (four ,6-diamidino-2diamidino-2-phenylindole); red color, GFAP. (Pictures: x200 magnification with the ipsilateral along with the phenylindole); red color, GFAP. (Pictures: x200 magnification of the ipsilateral and the contralateral contralateral hemispheres; scale bar = one hundred m) (B) The intensity of GFAP immunoreactive level hemispheres; scale bar = one hundred ) (B) The intensity of GFAP immunoreactive level with normalized with normalized intensity fluorescence unit in the 4 experimental groups is presented. (C) The intensity fluorescence constructive cells counterstained with DAPI within the four experimental groups is percentage of GFAP unit within the 4 experimental groups is presented. (C) The percentage of GFAP good cells counterstainedof GFAP in the corticalexperimental groups is presented. The expression presented. The expression with DAPI within the 4 injury internet site was calculated from six distinctive of GFAP levels.corticalwild-type sham; WT-T, wild-type with TBI; ARKO-S, ARKO sham; ARKO-T, bregma in the WT-S, injury web page was calculated from six distinctive bregma levels. WT-S, wild-type ARKO with wild-type with presented because the mean normal error. NS, no considerable distinction; sham; WT-T, TBI. All information areTBI; ARKO-S, ARKO sham; ARKO-T, ARKO with TBI. All data are p 0.05, and p 0.001; n = 7 in each and every NS, no presented as the imply standard error. group. considerable distinction; p 0.05, and p 0.001; n = 7 in each group.2.3. Effects of Androgen Receptor Knockout on Beclin-1 Expression in Mice Following TBI two.three. Effects of Androgen Receptor Knockout on Beclin-1 Expression in Mice following TBI Considering that autophagy plays a outstanding part in brain injury, we evaluated regardless of whether the Considering that receptor is involved in TBI-associated brain injury and autophagy. Figure 4A androgen autophagy plays a exceptional part in brain injury, we evaluated no matter whether the androgen.