The possibility of detecting good hits. This was specifically evident for the antibacterial and antidiabetic candidates that had been detectable only soon after fractionation. SPE fractionation also lowered the chemical variability of small molecules in every fraction, as a result simplifying the measures connected to the purification and identification of the active compounds. We focused the evaluation around the significant eukaryotic phyla so far explored in marine bioprospecting. An evaluation with the constructive hits confirmed sponges because the most prolific source of antitumoral and antibiotic activities, however it also recommended that other phyla are a lot more appropriate in addressing other therapeutic targets.Supplementary Materials: The following are accessible on-line at mdpi/article/10 .3390/md19110640/s1, Figure S1: Non-parametric test and Post-hoc evaluation: for each cell line (A2780, A549, PNT2) and dose (100, 10, 1 /mL), we computed the Kruskal allis test (non-parametric test). (1) Because the p-value was much less than the significance level 0.05, we can conclude that there are actually significant differences amongst the Phylum. (2) A several pairwise comparison involving 25-Hydroxycholesterol In stock Phylum is then performed to calculate pairwise comparisons in between Phylum levels, with corrections for several testing (Dunnett’s Test). (three) We show only the comparisons vs. CTRL; Figure S2. Barplot: Log Fold-Change for considerable comparisons (vs. CTRL-); Figure S3: Heatmap showing the percentage of vitality around the three cell lines (A549, A2780, PTN2) after therapy together with the raw extracts (X) and the corresponding 4 SPE fractions (B, C, D, E) at 1 and ten /mL; File S1 (cytotoxicity information); File S2 (antibacterial information); File S3 (antidiabetic data).Mar. Drugs 2021, 19,15 ofAuthor Contributions: Conceptualization, A.F. and G.N.; methodology, A.M.-F. and G.N.; formal evaluation, A.M.-F.; resources, A.S., V.M. and F.C.; information curation, E.M., G.d., J.H.A., M.A., A.I. (Antonella Iuliano), C.G. (Christian Galasso), C.S., F.C., G.B., C.G. (Carmela Gallo), A.I. (Adrianna Ianora) and G.N.; writing–original draft preparation, A.M.-F. and G.N.; writing–review and editing, A.F.; project administration, A.F.; funding acquisition, A.F. All authors have read and agreed for the published version in the manuscript. Funding: This operate was supported by the project “Antitumor Drugs and Vaccines in the Sea (ADViSE)” project (CUP B43D18000240007 URF 17061BP000000011) funded by POR Campania FESR 2014-2020 “Technology Platform for Therapeutic Approaches against Cancer”–Action 1.two.1 and 1.two.2. Acknowledgments: A.F. thanks Regione Campania for the generous assistance by means of the ADViSE project. Additionally, the authors thank Lucio Caso of ICB for the technical help. G.N. and a.F. thank Avila C., N��ez-Pons L. and Domenech A. for their assistance within the collection of some benthic organisms. Conflicts of Interest: The authors declare no conflict of interest.marine drugsReviewUntapped Prospective of Marine-Associated Cladosporium Species: An Overview on Secondary Metabolites, Biotechnological Relevance, and Biological ActivitiesGamal A. Mohamed 1, and Sabrin R. M. Ibrahim two,Division of Organic Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia Preparatory Year Program, Batterjee Zingerone medchemexpress Healthcare College, Jeddah 21442, Saudi Arabia; [email protected] Division of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt Correspondence: [email protected]; Tel.: 966-597-636-Citation: M.