Ric cancer EMT alterations and plays a crucial function in gastric cancer metastasiswere quantified by counting lesions in each sides of the lung per mouse. Representative images from tumors from…
F the trunk NC cells derived from bulk axial progenitor cultures (Figure 3B,C) originate from T-expressing cells. Similar to established in vitro neural induction methods, most current NC differentiation protocols…
R progression and tumor metastasis caused by reduced FBW7 expression in gastric cancer setting. In support of this hypothesis, we observed an inverse correlation of Brg1 and E-cadherin expression, a…
GRNA.AcknowledgementsWe would prefer to thank Katerina Politi (Yale University) for delivering gene expression information from her transgenic mice. We would like to thank members of your Varmus lab for helpful…
Ncer Pick siRNAs had been transfected employing Lipofectamine RNAiMAX (Invitrogen) following the manufacturer's advised protocol. Unless otherwise specified five nM of mimics, one hundred nM of anti-miRs or 40 nM…
Contain a subpopulation that displays a mixed early NC/NMP transcriptional signature and as a result is probably to represent the earliest trunk NC precursors. We demonstrate that T+ neuromesodermal potent…
H 2D and 3D culture of HepG2 cells confirmed that even though the HNF4-positive HepG2-cells didn't express BMAL1 (Supplementary Fig. 2a), HNF4 eficient Hepa-1c1c7 spheroids robustly expressed Bmal1 (Fig. 2b,…
Ipheral nervous program marker PERIPHERIN (PRPH) (Troy et al., 1990) collectively together with the trunk axial marker HOXC9 (Figure 5--figure supplement 1C). We also detected dramatic induction of GATA3, ASCL1,…
S41467-018-06038-y www.nature.com/naturecommunications1 KeyARTICLErg1, also called SMARCA4, encodes an ATPase subunit of the SWI/SNF chromatin remodeling complicated, which can shift the position of nucleosomes by utilizing the energy derived from ATP…
F the trunk NC cells derived from bulk axial progenitor cultures (Figure 3B,C) originate from T-expressing cells. Comparable to established in vitro neural induction approaches, most existing NC differentiation protocols…