Ds to many extracellular matrix (ECM) proteins, including laminin, fibrinogen, fibronectin
Ds to many extracellular matrix (ECM) proteins, including laminin, fibrinogen, fibronectin, nidogen-1, and endostatin, and to the proteoglycans, aggrecan and versican [12,13]. Mice lacking fibulin-1 die perinatally and display vascular anomalies in the kidney in addition to extensive hemorrhage in several organs, likely related to abnormalities in endothelial cell interactions with subendothelial ECM [14]. Fibulin-1 is reported to involve in the progression of many kinds of cancers, such as breast, ovarian PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28993237 and prostate cancer [15-18]. Besides, fibulin-1 has been identified epigenetically silenced in gastric cancer and hepatocellular carcinoma through promoter hypermethylation [19,20]. However, the association between fibulin-1 and bladder cancer remains unknown. Here we report that fibulin-1 expression is associated with NMIBC grade and recurrence, it is epigenetically down-regulated and functions as a tumor suppressor gene and angiogenesis inhibitor in bladder cancer.TURBT to the date of the first documented bladder tumor recurrence. The mean follow-up period for the study was 38 months (range, 33?3 months). There was no case of death in the study. The study protocol was approved by the Institutional Ethics Committee of Huazhong University of Science and Technology, Tongji Hospital and Chinese People’s Liberation Army General Hospital, and a written informed consent was obtained from all participants involved in the study.Cell culture and transfectionMuscle-invasive bladder cancer cell lines 5637, T24, J82 and HT1376 were purchased from ATCC and maintained in RPMI-1640 medium supplemented with 10 fetal bovine serum (FBS) in a humidified atmosphere of 5 CO2 maintained at 37 . SV-HUC-1 was also purchased from ATCC and maintained in DMEM/F-12 medium. Primary human umbilical vein endothelial cell (HUVEC) and Endothelial Cell Medium were purchased from ALLCELLS, LLC (Shanghai, China). Plasmid transfection was carried out using FuGene HD Transfection Reagent (Roche), according to the manufacture’s protocol.Plasmid, lentivirus and infectionGenerally, the full-length coding sequence (CDS) of FBLN1 was amplified from pBluescript-FBLN1 (Thermo scientific) and constructed into eukaryotic expression vector pEGFP-N1 (Clontech) or lentivirus clone vector pCDH-CMV. Then the lentivirus was packaged and purified according to the manufacture’s protocol (SBI, USA). The MOI for 5637 and HT1376 cells was 10 and 5 respectively.Immunohistochemical (IHC) stainingMethodsPatient and sample collectionThe cohort included 139 consecutive patients with NIMBC treated by TURBT in Chinese People’s Liberation Army General Hospital from December 2008 to September 2009. All tumors were initially staged, graded and classified by pathologists with expertise in genitourinary pathology according to 2002 TNM classification and 2004 WHO grading system. Adjuvant therapy of NIMBC after TURBT included intravesical instillation and chemotherapy. Followup information obtained from medical records of the patients who fulfilled inclusion criteria included tumor stage, grade, the development of tumor recurrence, the presence of multifocal versus unifocal tumor growth, the co-existence of CIS as well as patient gender and age. Briefly, patients were seen postoperatively at least every 3 to 4 months for the first 2 years and semiannually thereafter. Besides that, telephone follow up was taken every month. buy Roc-A recurrence was defined as a new tumor appearing in the bladder after initial cle.