Ceeds 50 [35] with P.falciparum accounting for .90 of cases [17]. Furthermore, Ghanaian patients exhibited a prevalence of mixed species including P.falciparum/P.malariae and P.falciparum/P.ovale, with at least P.ovale not being momelotinib custom synthesis present within South American parasite populations [16]. A recent study investigating HBV and Plasmodium co-infections in sub-Saharan Africa in an area with a similar prevalence of P.falciparum (.80 ) failed to demonstrate an association between HBV and Plasmodium infections, although a significant link with HCV was identified. Reduced differences observed between experimental groups may also reflect the asymptomatic status of patients included within the study, as observed previously [14]. With one or both 18334597 infections contained by the host immune system and in the absence of clinical pathology, this data may also suggest that there are no significant interactions between the two pathogens. The data presented in this study of 117 hospitalized patients asymptomatic for both HBV and malaria confirmed HBV epidemiologic predictions, since 90.3 had been exposed to HBV although 42.2 instead of the predicted 20 of patients had active HBV infection defined by the presence of viral DNA in plasma. The difference in prevalence of viremia might be in part related to data from blood donors being collected in a younger population, a fifth of whom had previously tested negative for HBsAg (repeat donors). It may also be related in part to the use of a more sensitive assay for HBV DNA detection reflected in the prevalence of HBsAg negative/HBV DNA positive occult HBV infections found at 1.6 in blood donors but 7.1 in patients. In a population of pregnant women in Kumasi, 16 had active infections but only 1.5 were occult [23]. In this latter study, 5.4 of HBsAg positive samples were HBV DNA negative while in the present study only 2.3 were DNA negative, strongly suggesting an increased sensitivity of HBV DNA detection. In addition, the phylogenetic analysis indicated that all sequenced samples were genotype E, confirming previous reports [23,36].The prevalence and molecular epidemiology of Plasmodium presented here also supported previous reports (50 and 55 , respectively) [35] with the majority of parasitemic recipients presenting single species P.falciparum infection. Data also confirmed that parasitemic individuals were significantly younger than nonparasitemic recipients (Figure 3), confirming similar observations CX-5461 web reported in the literature [37,38]. This is likely to reflect the slow development of semi-immunity efficacy, typically 1407003 observed in older individuals who reside in areas with high transmission intensity [39].Figure 3. Age distribution of Ghanaian transfusion recipients stratified according to HBV and malaria parasitemia status. Number of samples included = 117. HBV negative (N = 68); HBV positive (N = 49); Plasmodium negative (N = 59); Plasmodium positive (N = 58); HBV positive/Plasmodium negative (N = 20); HBV positive/Plasmodium positive (N = 29). *: Age distribution of parasitemic and non-parasitemic patients (Mann-Whitney, P = 0.0397). doi:10.1371/journal.pone.0049967.gImpact of Hepatitis B on Plasmodium InfectionsWhilst steps have been taken to minimize the impact of host genetic and external factors i.e. other pathogens, these efforts were not exhaustive. Despite the heterogeneous nature of clinical complications suffered by patients included in this study, none presented clinical conditions relatin.Ceeds 50 [35] with P.falciparum accounting for .90 of cases [17]. Furthermore, Ghanaian patients exhibited a prevalence of mixed species including P.falciparum/P.malariae and P.falciparum/P.ovale, with at least P.ovale not being present within South American parasite populations [16]. A recent study investigating HBV and Plasmodium co-infections in sub-Saharan Africa in an area with a similar prevalence of P.falciparum (.80 ) failed to demonstrate an association between HBV and Plasmodium infections, although a significant link with HCV was identified. Reduced differences observed between experimental groups may also reflect the asymptomatic status of patients included within the study, as observed previously [14]. With one or both 18334597 infections contained by the host immune system and in the absence of clinical pathology, this data may also suggest that there are no significant interactions between the two pathogens. The data presented in this study of 117 hospitalized patients asymptomatic for both HBV and malaria confirmed HBV epidemiologic predictions, since 90.3 had been exposed to HBV although 42.2 instead of the predicted 20 of patients had active HBV infection defined by the presence of viral DNA in plasma. The difference in prevalence of viremia might be in part related to data from blood donors being collected in a younger population, a fifth of whom had previously tested negative for HBsAg (repeat donors). It may also be related in part to the use of a more sensitive assay for HBV DNA detection reflected in the prevalence of HBsAg negative/HBV DNA positive occult HBV infections found at 1.6 in blood donors but 7.1 in patients. In a population of pregnant women in Kumasi, 16 had active infections but only 1.5 were occult [23]. In this latter study, 5.4 of HBsAg positive samples were HBV DNA negative while in the present study only 2.3 were DNA negative, strongly suggesting an increased sensitivity of HBV DNA detection. In addition, the phylogenetic analysis indicated that all sequenced samples were genotype E, confirming previous reports [23,36].The prevalence and molecular epidemiology of Plasmodium presented here also supported previous reports (50 and 55 , respectively) [35] with the majority of parasitemic recipients presenting single species P.falciparum infection. Data also confirmed that parasitemic individuals were significantly younger than nonparasitemic recipients (Figure 3), confirming similar observations reported in the literature [37,38]. This is likely to reflect the slow development of semi-immunity efficacy, typically 1407003 observed in older individuals who reside in areas with high transmission intensity [39].Figure 3. Age distribution of Ghanaian transfusion recipients stratified according to HBV and malaria parasitemia status. Number of samples included = 117. HBV negative (N = 68); HBV positive (N = 49); Plasmodium negative (N = 59); Plasmodium positive (N = 58); HBV positive/Plasmodium negative (N = 20); HBV positive/Plasmodium positive (N = 29). *: Age distribution of parasitemic and non-parasitemic patients (Mann-Whitney, P = 0.0397). doi:10.1371/journal.pone.0049967.gImpact of Hepatitis B on Plasmodium InfectionsWhilst steps have been taken to minimize the impact of host genetic and external factors i.e. other pathogens, these efforts were not exhaustive. Despite the heterogeneous nature of clinical complications suffered by patients included in this study, none presented clinical conditions relatin.