Shi Y, et al. An enhanced U6 promoter for synthesis of short hairpin RNA. Nucleic Acids Res 31: e100. 16. Tiscornia G, Tergaonkar V, CB-5083 Galimi F, Verma IM CRE INCB-039110 recombinaseinducible RNA interference mediated by lentiviral vectors. Proc Natl Acad Sci U S A 101: 73477351. 17. Kohfeldt E, Maurer P, Vannahme C, Timpl R Properties of your extracellular calcium binding module of your proteoglycan testican. FEBS Lett 414: 557561. 18. Pepperkok R, Squire A, Geley S, Bastiaens PI Simultaneous detection of several green fluorescent proteins in reside cells by fluorescence lifetime imaging microscopy. Curr Biol 9: 269272. 19. de la Luna S, Ortin J pac gene as effective dominant marker and reporter gene in mammalian cells. Strategies Enzymol 216: 376385. 20. Deuschle U, Meyer WK, Thiesen HJ Tetracycline-reversible silencing of eukaryotic promoters. Molecular and cellular biology 15: 19071914. 21. Pear WS, Nolan GP, Scott ML, Baltimore D Production of high-titer helper-free retroviruses by transient transfection. Proc Natl Acad Sci U S A 90: 83928396. 22. Csordas A, Kreutmayer S, Ploner C, Braun PR, Karlas A, et al. Cigarette smoke extract induces prolonged endoplasmic reticulum pressure and autophagic cell death in human umbilical vein endothelial cells. Cardiovascular study 92: 141148. 23. Ploner C, Rainer J, Niederegger H, Eduardoff M, Villunger A, et al. The BCL2 rheostat in glucocorticoid-induced apoptosis of acute lymphoblastic leukemia. Leukemia: official journal with the Leukemia Society of America, Leukemia Research Fund, UK 22: 370377. 24. Tugendreich S, Boguski MS, Seldin MS, Hieter P Linking yeast genetics to mammalian genomes: identification and mapping in the human homolog of CDC27 via the expressed sequence tag data base. Proc Natl Acad Sci U S A 90: 1003110035. 25. Wirth KG, Ricci R, Gimenez-Abian JF, Taghybeeglu S, Kudo NR, et al. Loss of the anaphase-promoting complex in quiescent cells causes unscheduled hepatocyte proliferation. Genes Dev 18: 8898. 26. Sigl R, Wandke C, Rauch V, Kirk J, Hunt T, et al. Loss from the mammalian APC/C activator FZR1 shortens G1 and lengthens S phase but has tiny effect on exit from mitosis. Journal of cell science 122: 42084217. 27. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, et al. Enhanced monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22: 15671572. 28. Donnelly ML, Luke G, Mehrotra A, Li X, Hughes LE, et al. Evaluation with the aphthovirus 2A/2B polyprotein `cleavage’ mechanism indicates not a proteolytic reaction, but a novel translational impact: a putative ribosomal `skip’. J Gen Virol 82: 10131025. 29. Kim JH, Lee SR, Li LH, Park HJ, Park JH, et al. High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. PLoS One six: e18556. 30. Jackson AL, Bartz SR, Schelter J, Kobayashi SV, Burchard J, et al. Expression profiling reveals off-target gene regulation by RNAi. Nat Biotechnol 21: 635637. 31. Pei Y, Tuschl T Around the art of identifying helpful and particular siRNAs. Nature solutions three: 670676. 32. Birmingham A, Anderson EM, Reynolds A, Ilsley-Tyree D, Leake D, et al. 3′ UTR seed matches, but not all round identity, are related to RNAi off-targets. Nature approaches three: 199204. 33. Bulliard Y, Wiznerowicz M, Barde I, Trono D KRAB can repress lentivirus proviral transcription independently of integration site. J Biol Chem 281: 3574235746. eight ~~ ~~ Osteoporotic fractures.Shi Y, et al. An enhanced U6 promoter for synthesis of short hairpin RNA. Nucleic Acids Res 31: e100. 16. Tiscornia G, Tergaonkar V, Galimi F, Verma IM CRE recombinaseinducible RNA interference mediated by lentiviral vectors. Proc Natl Acad Sci U S A 101: 73477351. 17. Kohfeldt E, Maurer P, Vannahme C, Timpl R Properties from the extracellular calcium binding module with the proteoglycan testican. FEBS Lett 414: 557561. 18. Pepperkok R, Squire A, Geley S, Bastiaens PI Simultaneous detection of several green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy. Curr Biol 9: 269272. 19. de la Luna S, Ortin J pac gene as efficient dominant marker and reporter gene in mammalian cells. Procedures Enzymol 216: 376385. 20. Deuschle U, Meyer WK, Thiesen HJ Tetracycline-reversible silencing of eukaryotic promoters. Molecular and cellular biology 15: 19071914. 21. Pear WS, Nolan GP, Scott ML, Baltimore D Production of high-titer helper-free retroviruses by transient transfection. Proc Natl Acad Sci U S A 90: 83928396. 22. Csordas A, Kreutmayer S, Ploner C, Braun PR, Karlas A, et al. Cigarette smoke extract induces prolonged endoplasmic reticulum pressure and autophagic cell death in human umbilical vein endothelial cells. Cardiovascular research 92: 141148. 23. Ploner C, Rainer J, Niederegger H, Eduardoff M, Villunger A, et al. The BCL2 rheostat in glucocorticoid-induced apoptosis of acute lymphoblastic leukemia. Leukemia: official journal in the Leukemia Society of America, Leukemia Investigation Fund, UK 22: 370377. 24. Tugendreich S, Boguski MS, Seldin MS, Hieter P Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 by means of the expressed sequence tag data base. Proc Natl Acad Sci U S A 90: 1003110035. 25. Wirth KG, Ricci R, Gimenez-Abian JF, Taghybeeglu S, Kudo NR, et al. Loss from the anaphase-promoting complicated in quiescent cells causes unscheduled hepatocyte proliferation. Genes Dev 18: 8898. 26. Sigl R, Wandke C, Rauch V, Kirk J, Hunt T, et al. Loss of your mammalian APC/C activator FZR1 shortens G1 and lengthens S phase but has little effect on exit from mitosis. Journal of cell science 122: 42084217. 27. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22: 15671572. 28. Donnelly ML, Luke G, Mehrotra A, Li X, Hughes LE, et al. Analysis of your aphthovirus 2A/2B polyprotein `cleavage’ mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal `skip’. J Gen Virol 82: 10131025. 29. Kim JH, Lee SR, Li LH, Park HJ, Park JH, et al. Higher cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. PLoS One particular 6: e18556. 30. Jackson AL, Bartz SR, Schelter J, Kobayashi SV, Burchard J, et al. Expression profiling reveals off-target gene regulation by RNAi. Nat Biotechnol 21: 635637. 31. Pei Y, Tuschl T On the art of identifying helpful and certain siRNAs. Nature procedures three: 670676. 32. Birmingham A, Anderson EM, Reynolds A, Ilsley-Tyree D, Leake D, et al. 3′ UTR seed matches, but not all round identity, are linked to RNAi off-targets. Nature approaches 3: 199204. 33. Bulliard Y, Wiznerowicz M, Barde I, Trono D KRAB can repress lentivirus proviral transcription independently of integration internet site. J Biol Chem 281: 3574235746. 8 ~~ ~~ Osteoporotic fractures.