10,0006g, 4uC for 10 min and the supernatant was used as crude Celgosivir site enzyme preparation. Laccase activity was determined using 2 mM guaiacol, at 55uC in 0.1 26225771 M phosphate buffer . The change in absorbance due to oxidation of substrate in the reaction mixture was monitored after 10 min of incubation at 465 nm. Unit of laccase was expressed in nkat. Pulp Preparation Waste papers were shredded and soaked overnight in tap water at room temperature. The soaked papers were washed several times and were disintegrated with the help of a grinder to obtain soft cottony pulp. It was then squeezed to remove absorbed water and oven dried. This oven dried pulp was used for further experiments. Materials and Methods Wastepaper Pulps The different pulps used were made from locally procured wastepapers viz. old newsprint, magazines, laser, inkjet and xerox with initial brightness of 36, 42, 65, 60 and 58% ISO respectively. Pulp Deinking Sequences All treatments were carried out in triplicates at least. A standard deinking sequence was performed in the laboratory. P refers to the pulp preparation stage and C refers to chemical bleaching stage where pulp at 5% consistency was treated with sodium hydroxide, sodium silicate, DTPA, Triton X-100 
and H2O2 at 50uC and 200 rpm for 15 min. To facilitate removal of ink particles detached from fibers, pulp 26507655 was thoroughly washed with distilled water and was used to make hand sheets to determine brightness of the pulp. Enzymatic and physical pretreatments were evaluated using the standard sequence by incorporating xylanase stage, laccase stage, L stage along with Enzymes A cellulase-free, thermo-alkali-stable and halo-tolerant xylanase with optimum activity at pH 89.5 and 65uC was produced from Bacillus halodurans FNP 135 soil isolate by solid state fermentation of wheat bran under optimized conditions viz. 2.31% Na2CO3, 80% moisture content, 68 h of fermentation at 37uC and assayed using birchwood xylan as substrate. In all cases, standard sequence was used as a control. Both xylanase and laccase treatments were carried out with 10 g of pulp at 5% consistency at different enzyme doses, temperatures and pH for optimization of various parameters for deinking using Response Surface Methodology. The treatments were carried out in flasks, at 160 rev/min and 65uC and 55uC for xylanase and laccase respectively. Laccase mediator and inhibitor treatments were carried out with 10 g of pulp at 5% consistency at optimum pH, enzyme dose and incubation time using 2 mM of 2,29-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid, 1-hydroxybenzotriazole, syringaldehyde as mediators and 1 mM of sodium azide as inhibitor. Sonication was carried out at 1% consistency, 20 kHz and microwaving was carried out at 20% consistency, 850 W employing optimum pH, enzyme dose and incubation time for different time intervals. Analysis of Collected Filtrate The color removal from the pulp was determined spectrophotometrically from l 200 nm to l 800 nm. The reducing sugar released was measured by the dinitrosalicylic acid , phenolic compounds generated were estimated by measuring the absorbance at l 237 nm and release of hydrophobic compounds were determined by measuring the absorbance at l 465 nm. X-ray Diffraction Fourier Transformed Infrared Spectroscopy FTIR spectra for pulp samples were recorded with a resolution of 4 cm21 over the wave number range of 4000400 cm21, using 32 scans per sample. Scanning Electron Microscopy of Pulp Fibers The fibers were wa