Intracellular alkaline phosphatase (ALP) action of ccMSCs was calculated right after 21 days employing mobile lysate calculated colorimetrically with p-nitrophenyl phosphate as a substrate (Sigma)
Floating cells have been gathered and adherent cells have been detached by incubation with Trypsin-EDTA for 2 minutes. Cells were washed and re-suspended in five hundred L of Annexin V…